RPMI Medium 1640 solarbio 細胞培養(yǎng)

貨號：90022
規(guī)格：500ml
儲存條件：2-8° C，避光保存，有效期 12 個月
單位：瓶

細胞凋亡與細胞壞死不同，細胞凋亡是為維持環(huán)境穩(wěn)定，為更好的適應(yīng)勝春環(huán)境而主動爭取的一種死亡過程，它涉及一系列基因的激活、表達以及調(diào)控等作用，是一種字體損傷的現(xiàn)象。

細胞凋亡檢測試劑盒原理

細胞凋亡是細胞的基本特征之一，在機體的胚胎發(fā)育、組織修復(fù)、內(nèi)環(huán)境的穩(wěn)定等方面都起到十分重要的作用。Annexin V是一種分子量為35.8 KD的Ca2+依賴性磷脂結(jié)合蛋白，能與細胞凋亡過程中翻轉(zhuǎn)到膜外的磷脂酰絲氨酸（Phosphatidylserine, PS）高親和力特異結(jié)合。FITC-Annexin V結(jié)合到凋亡細胞后，在藍色光的激發(fā)下，發(fā)出綠色熒光，區(qū)分出凋亡細胞與正常細胞。碘化丙啶（Propidium iodide，PI）是一種核酸染料，它不能穿透完整細胞膜，但對凋亡晚期細胞和死細胞的破損細胞膜能夠穿透，并使細胞核紅染。將FITC-Annexin V與PI匹配使用，可以將凋亡早期的細胞和晚期的細胞區(qū)分開來。

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RPMI Medium 1640 solarbio 細胞培養(yǎng)